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Figure 1 | Virology Journal

Figure 1

From: Functional analysis of the interferon-stimulated response element of porcine circovirus type 2 and its role during viral replication in vitro and in vivo

Figure 1

Function analysis of the PCV2 ISRE in the context of an enhancer test vector. The pGm-PCV2-ISRE(3), pGm-PCV2-ISREm(3) and pGm-GPB-ISRE(3) constructs contain the PCV2 ISRE, mutant PCV2 ISRE and GBP2 promoter ISRE double-stranded oligonucleotides cloned into the minimal promoter construct pGL-miniP, respectively. The number of copies of the oligonucleotide in the constructs is shown in parenthese in the construct name. Luciferase activity was measured in extracts of transfected cells treated with porcine IFN-α, and compared with untreated cells. pRL-TK was included in every transfection mixture to control for variations in transfection efficiencies. The level of Renila luciferase in each sample was used to normalize firefly luciferase activity. The final concentration of porcine IFN-α was 100 U/mL. Experiments were carried out independently at least 3 times and the results are expressed as means ± SD.

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Virology Journal

ISSN: 1743-422X