Figure 6
Effect of agents that inhibit endocytic virion uptake or low pH-dependent infection. (A) CHO-A and Vero cells were incubated with KOS/tk12 (MOI = 3) in hypertonic medium containing 0.3 M sucrose for 1 h at 4°C and infections were performed in the continuous presence of sucrose followed by ONPG assay. (B) CHO-K1 cells were incubated with KOS/tk12 (MOI = 3) in hypertonic medium containing 0.3 M sucrose for 1 h at 4°C, sHA102 was added to 500 nM, and infections were performed in the continued presence of sucrose followed by ONPG assay. (C, D) CHO-K1 cells and CHO-HVEM-12 cells (CHO-A) were pretreated with the indicated amounts of NH4Cl (C) or monensin (D) for 30 min at 4°C prior to KOS/tk12 attachment at 4°C (MOI = 3), addition of sHA102 to 500 nM (CHO-K1 cells only), and infection as in previous figures in the continued presence of the respective drugs. Infection was measured by ONPG assay. Values are averages of triplicate determinations.