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Table 2 Analysis of percentage of apoptotic cells in control, mock transfected and NS1 transfected cells as observed in TUNEL assay experiment

From: Non structural protein of avian influenza A (H11N1) virus is a weaker suppressor of immune responses but capable of inducing apoptosis in host cells

Constructs

Percentage (%) of positive cells per field out of 106cells used in TUNEL assay

Control

0

Vector (pcDNA3.0)

0

H5N1-NS1

40%

H11N1-NS1

20%

  1. The cells were analyzed 24 h post transfection using fluorescently labeled anti-BrdU antibodies. The fluorescence was detected microscopically. The percentage of fluorescent cells is represented as the degree of apoptosis in transfected cells.