Table 2 Rt-PCR detection of H5N1 in aerosols generated by various experimental procedures
From: Aerosolized avian influenza virus by laboratory manipulations
| Â | Group I: zootomy | Group II: grinding | Group III: centrifugation | Group IV: pipetting | Group V: magnetic stirring | Group VI: egg inoculation | Group VII: zoogenetic infection |
|---|---|---|---|---|---|---|---|
Collection time, h | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
Temperature,°C | 29.5 ± 3.6 | 30.6 ± 1.7 | 28.1 ± 1.0 | 25.2 ± 1.9 | 28.0 ± 2.1 | 30.8 ± 0.9 | 30.9 ± 1.9 |
Relative humidity,% | 28.0 ± 5.1 | 22.8 ± 2.2 | 25.6 ±1.3 | 30.5 ± 3.3 | 26.1 ± 3.2 | 22.3 ± 1.3 | 21.9 ± 2.4 |
Positive experimental samples, n/total | 6/6 | 6/6 | 0/3 | 3/3 | 3/3 | 3/3 | 3/3 |
Positive of control samples, n/total | 0/6 | 0/6 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |