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Figure 4 | Virology Journal

Figure 4

From: Avian influenza virus H5N1 induces rapid interferon-beta production but shows more potent inhibition to retinoic acid-inducible gene I expression than H1N1 in vitro

Figure 4

H5N1 infection or NS1 overexpression showed potent inhibition to exogenous IFN. ( A) HEK293T cells were pre-infected as indicated. At 4 h post infection, the cells were treated with 2000 IU/ml of hIFNα2b (InvivoGen, San Diego, CA) for the indicated time. The expression of endogenous RIG-I and NS1 were analyzed with western blot, beta-actin as loading control. ( B) HEK293T cells were co-transfected with the ISRE-luc reporter gene, pCMV-renilla, and NS1 constructs. At 16 h post transfection, cells were treated with 2000 IU/ml of hIFNa2b for 8 h. The cells were collected, and luciferase reporter activity was measured with the Dual-Luciferase Reporter Assay System. Fold induction is the ratio of the activity induced by the hIFNa2b treatment to that of the untreated control. In all cases, data are shown as mean ± SD of triplicate samples. The expression level of NS1 constructs in HEK293T was shown in western blot and measured with Scion image software (National Institute Health).

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Virology Journal

ISSN: 1743-422X