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Figure 3 | Virology Journal

Figure 3

From: Avian influenza virus H5N1 induces rapid interferon-beta production but shows more potent inhibition to retinoic acid-inducible gene I expression than H1N1 in vitro

Figure 3

Involvement of RIG-I in recognizing influenza virus. ( A) The A549 cells were cotransfected with the IFN-luc reporter gene, pCMV-renilla, and RIG-I-flag K270A. At 16 h post transfection, the cells were infected with the H5N1 or H1N1 (m.o.i = 2). At 12 h post infection, the cells were collected and luciferase reporter activity was measured with the Dual-Luciferase Reporter Assay System. In all cases, the data are shown as mean ± SD of triplicate samples of a representative from three independent experiments. ( B) Overexpressing RIG-I increases STAT-1 phosphorylation induced by H1N1 or H5N1 infection. HEK293T cells were transfected with RIG-I flag. At 16 h posttransfection, cells were infected with indicated influenza virus (m.o.i = 2). Then the cells were collected at 4 h and 8 h post infection, and western blot was used to detect STAT-1 phosphorylation, total STAT-1, RIG-I, and NS1 expression.

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Virology Journal

ISSN: 1743-422X