Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch

Table 1 Primer comparison between Gouvea et al. and Iturriza-Gomara et al. Note that primer for G3 and G9 are different

Primers of Gouvea et al. (Set A)		Primers of Iturriza-Gomara et al. (Set B)
Primer name	Primer sequence	Primer name	Primer sequence
aBT1	CAAGTACTCAAATCAATGATGG	G1	CAAGTACTCAAATCAATGATGG
aCT2	CAATGATATTAACACATTTTCTGTG	G2	CAATGATATTAACACATTTTCTGTG
aET3	CGTTTGAAGAAGTTGCAAG	G3	ACGAACTCAACACGAGAG
aDT4	CGTTTCTGGTGAGGAGTTG	G4	CGTTTCTGGTGAGGGTTG
aFT9	CTAGATGTAACTACAACTC	G9	CTTGATGTGACTAYAAATAC

Primers for G1, G2 and G4 in Iturriza-Gomara et al. were adapted from primers of Gouvea et al.

ISSN: 1743-422X