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Figure 3 | Virology Journal

Figure 3

From: Free fatty acids induce ER stress and block antiviral activity of interferon alpha against hepatitis C virus in cell culture

Figure 3

Effect of hepatocellular steatosis on HCV RNA replication in the infected Huh 7.5 cells. Huh-7.5 cells were infected overnight with HCV chimeric virus encoding Renilla luciferase (pJFH-ΔV3-Rluc). Infected Huh-7.5 cells were cultured long-term in growth media by splitting at a 1:10 ratio at five days intervals. (A). HCV-Renilla luciferase activity of infected Huh-7.5 cells cultured in the presence FFA for 72 hrs. The values in the y-axis represent the normalized Renilla luciferase values per one μg of total protein. The X-axis shows the different concentrations of FFA. (B). Effect of long-term culture FFA on HCV replication. HCV infected cells were cultured in the presence of 100 μM of FFA for 5, 10 and 15 days, the level of HCV RNA was measured by luciferase assay. The statistical significance was determined by paired T-test, the stars indicate a p value <0.05. (C). HCV infected cells were cultured in the presence of 100 μM concentrations of FFA for 5, 10 and 15 days. HCV replication was measured by luciferase assay. The statistical significance was determined by paired T-test, the stars indicate a p value <0.05. (D) Immunohistochemical staining for HCV core antigen in the infected Huh 7.5 cells in the presence of different concentrations of FAA after 15 days.

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