Figure 2

Effect of hepatocellular steatosis on HCV RNA replication in S3-GFP replicon cell line. S3-GFP replicon cells were cultured with different concentrations of FFA in 1% BSA for 5 days. Untreated cells received only 1% BSA. (A) The HCV-GFP expression was measured by using fluorescence microscopy. (B). Quantification of GFP expression of S3-GFP in replicon cells by flow analysis. The number indicates the mean fluorescence of GFP-positive cells. (C). Intracellular HCV RNA levels in S3-GFP replicon cells were measured by real time RT-PCR.