Figure 6From: Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cellViral growth curve analysis. Marc-145 cells in T-25 flasks were infected with PRRSV nsp2 small deletion mutants and parental virus at 100TCID 50 . The cell supernatants were collected for titration analysis by the method of Reed-Muench at every 12 h hpi. The virus was titrated on Marc-145 cells.Back to article page