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Figure 4 | Virology Journal

Figure 4

From: Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell

Figure 4

Identification of the deletion or insertion viruses by IFA test. Marc-145 cells were infected with the five passages deletion viruses, rHuN4-F112-Δ 480-532, rHuN4-F112-Δ 508-532, rHuN4-F112-Δ 480-620 rHuN4-F112-Δ 480-667 and parental virus rHuN4-F112. Approximately 24 h after infection, cells were processed for indirect immunofluorescence after incubation with Abs specific for PRRSV NP protein (Anti-NP Ab), nsp4 protein (Anti-nsp4 Ab). As expected, the Ab specific for NP protein and nsp4 protein could react with all these 5 recovery viruses.

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Virology Journal

ISSN: 1743-422X