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Table 2 RT-PCR and RT-qPCR from L1-dsRNAs isolated from different  X. dendrorhous  strains

From: Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous

Strain

Primers pairs

Ratioa L1B/L1A

 

Mot4F Mot5R

Mot4F Mot6R

L8.2R SR4

LIVR3 Cap288Rv

Mot4FB Mot5RB

Mot4FB Mot6RB

L1BF L1BR

L1AF L1AR

 

VKM Y-2059

+

+

-

-

+

+

-

-

nd

UCD 67–202

+

+

+

+

+

+

-

-

nd

VKM Y-2786

+

+

-

-

+

+

-

-

nd

UCD 68-653 C*

-

-

-

-

-

-

nd

nd

nd

UCD 67–385

+

+

+

+

+

+

+

+

14 (6)

385(S2)-40

+

+

+

+

+

+

+

+

0.4 (0.2)

  1. RT-PCR determinations were made at least 3 times for each dsRNA. *, L2 dsRNA was analyzed. +: an amplicon of the expected size; -: no amplicon; nd: not determined. a, ratios were calculated from ct values obtained through RT-qPCR, the average of three biological replicates with three technical replicates are given; the standard deviations are shown in parenthesis.