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Table 2 RT-PCR and RT-qPCR from L1-dsRNAs isolated from different  X. dendrorhous  strains

From: Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous

Strain Primers pairs Ratioa L1B/L1A
  Mot4F Mot5R Mot4F Mot6R L8.2R SR4 LIVR3 Cap288Rv Mot4FB Mot5RB Mot4FB Mot6RB L1BF L1BR L1AF L1AR  
VKM Y-2059 + + - - + + - - nd
UCD 67–202 + + + + + + - - nd
VKM Y-2786 + + - - + + - - nd
UCD 68-653 C* - - - - - - nd nd nd
UCD 67–385 + + + + + + + + 14 (6)
385(S2)-40 + + + + + + + + 0.4 (0.2)
  1. RT-PCR determinations were made at least 3 times for each dsRNA. *, L2 dsRNA was analyzed. +: an amplicon of the expected size; -: no amplicon; nd: not determined. a, ratios were calculated from ct values obtained through RT-qPCR, the average of three biological replicates with three technical replicates are given; the standard deviations are shown in parenthesis.