Table 1 Oligonucleotides used in this work
From: Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous
Name | Position or application | Sequence (5’-3’) |
|---|---|---|
NBA1 | Tailing of dsRNAs | (PO4)-GCAATTGTACGCCTGGAGCGC-(NH2) |
NBA-1 | Complementary to NBA1 | GCGCTCCAGGCGTACAATTGCG |
Mot4F | Conserved motif 4 of RdRp of XdV-L1A | GAGGACTTCAATAGTCAACA |
Mot5R | Conserved motif 5 of RdRp of XdV-L1A | AAGTCGTCAGCCTCCACCCC |
Mot6R | Conserved motif 6 of RdRp of XdV-L1A | AGACATCGTCTCCGTTGTGC |
L8.2R | CP ORF of XdV-L1A | CCGTGTAGAGCTAAAATACC |
SR4 | CP ORF of XdV-L1A | AAGCGCATCTTCTGGGCTCA |
LIVR3 | CP ORF of XdV-L1B | CCTCTGCTGATCTGAAATGTT |
Cap288R | CP ORF of XdV-L1B | GAAGTTTTCGCCCACAAGAG |
Mot4FB | Conserved motif 4 of RdRp of XdV-L1B | GAAGATTTCAACAGTCAACATAG |
Mot5RB | Conserved motif 5 of RdRp of XdV-L1B | ATGTCGTTAACCTCCAGCCC |
Mot6RB | Conserved motif 6 of RdRp of XdV-L1B | GCACGTCGTCGCCGTTATG |
L1AF | RT-qPCR, specific for XdV-L1A | GCGAAAATGAAGCGATGACA |
L1AR | RT-qPCR, specific for XdV-L1A | TTAGTCTCCGCGCCCTTCTA |
L1BF | RT-qPCR, specific for XdV-L1B | GACGAACTGATGCCCAAACA |
L1BR | RT-qPCR, specific for XdV-L1B | CCGGAGACAGCUCAUUGUA |
Random hexamer | Random cloning | NNNNNN |
M13F | Vector | GTAAAACGACGGCCAGT |
M13R | Vector | AACAGCTATGACCATG |