Table 2 Strains and plasmids used in this study
From: The Rift Valley Fever virus protein NSm and putative cellular protein interactions
Yeast strain | Genotype | ||
|---|---|---|---|
MATα, ura3-52, his3-200, ade2-101, trp1-901, leu2-3, | |||
Y187 | 112, gal4Δ, met - , gal80Δ, URA3::GAL1 UAS -GAL1 TATA -lacZ, MEL1 | ||
MATa, ura3-52, his3-200, trp1-901, leu2-3, | |||
AH109 | 112, gal4Δ, gal80Δ, LYS2::GAL1 UAS -GAL1 TATA -HIS3, MEL1 | ||
GAL2 UAS -GAL2 TATA -ADE2, URA3::MEL1 UAS -MEL1 TATA -lacZ | |||
Plasmid | Size (kb) | Selectable marker on SD-media | Additional information |
pGADT7 | 8.0 | -Leu | GAL4(768–881)AD, LEU2, ampr, HA epitope tag |
pGBKT7 | 7.3 | -Trp | GAL4(1–147)DNA-BD, TRP1, kanr, c-Myc epitope tag |