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Figure 2 | Virology Journal

Figure 2

From: Visual detection of the human metapneumovirus using reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye

Figure 2

Specificity and sensitivity of hMPV RT-LAMP. (A) HRV, influenza virus A/PR/8/34 (H1N1), and RSV positive nucleic were amplified by RT-LAMP using the primers as in Figure 4. The RT-LAMP products were tested by agarose gel electrophoresis (upper panel) and direct color change (lower panel). Lane M, DNA marker DL2000 (Biomed, China, with bands of 2000, 1000, 750, 500, 250 and 100 bp); 1, positive hMPV RNA; 2, influenza virus A/PR/8/34; 3, RSV; 4, HRV; 5, DEPC-treated H2O. (B) RNA serially diluted was amplified and the RT-LAMP products were tested by two methods as above. Lane M, DNA marker (as above); 1, 105 copies; 2, 104 copies; 3, 103 copies; 4, 102 copies; 5, 10 copies; 6, Single copy; 7, DEPC-treated H2O.

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