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Figure 1 | Virology Journal

Figure 1

From: Visual detection of the human metapneumovirus using reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye

Figure 1

hMPV RT-LAMP amplification and digestion of positive RT-LAMP products. (A) RT-LAMP products were examined by agarose gel electrophoresis (upper panel) and visually amplification for color change (lower panel). Lane M, DNA marker DL2000 (Biomed, China, with bands of 2000, 1000, 750, 500, 250 and 100 bp); 1–2, positive hMPV RNA; 3, DEPC-treated H2O. (B) RT-LAMP products were digested with Msp I, and two fragments (103 bp, 116 bp) were observed by polyacrylamide gel electrophoresis attained with ethidium bromide and photographed under a UV transilluminator. 1, RT-LAMP products without digestion; 2, RT-LAMP products digested by Msp I; M, DNA marker DL500 (Biomed, China, with bands of 500, 400, 300, 250, 200, 150, 100 and 50 bp).

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Virology Journal

ISSN: 1743-422X