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Table 4 Monoclonal antibodies characterization and testing of passive protection

From: Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model

MAbs Intracellulara Surface associatedb IFAc Affinity KD (nM)d MAb binding domaine % Mice survival ratef
    JEV Dengue 2 V    
3E10 + + + - 1.1 unknown 16
4 C4 + + + + 56 NS11–143 33
7 C2 + + + + 17 NS1224–352 8
7 H5 + + + + 6.4 NS1224–352 0
8 F1 + + + + 5 NS1224–352 0
  1. a/bMAbs were incubated with S2-NS11–352 cells permeabilized or untreated (intracellular/surface associate) for fluorescence testing by flow cytometry (FACScan).
  2. c Immunofluorescence assay (IFA) for testing monoclonal antibodies reactivity against BHK cells infected with JEV, and C6/36 cells infected with Dengue 2 virus.
  3. d The affinity of MAbs to NS1 tested by surface plasmon resonance (SPR).
  4. e Reactivity of MAbs for S2- NS11–143 or NS1224–352 fragments. Cells lysates were tested by Western blotting (WB). “Unknown” means did not react with both of the NS1 fragments or reduced and boiled NS1.
  5. f Six to twelve mice were administered with 100μg of 3E10, 4 C4, 7 C2 and 8 F1, respectively, and challenged by JEV SA14.