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Table 2 Relative quantitative real-time PCR primers for targeted transcripts mRNAs

From: Induction of antigen-specific immune responses in mice by recombinant baculovirus expressing premembrane and envelope proteins of West Nile virus

Targeted transcriptsa

Primer sequence (5’to 3’)

Conditions

Forward

Reverse

β-actinb

CACTGCCGCATCCTCTTCCTCCC

CAATAGTGATGACCTGGCCGT

50°C 2min; 94°C 10min 40 cycles (94°C 15s then 60°C 1min)

IFN-γ

TCAAGTGGCATAGATGTGGAAGAA

TGGCTCTGCAGGATTTTCATG

 

TNF-α

TGTCTCAGCCTCTTCTCATTCC

TTAGCCCACTTCTTTCCCTCAC

 

IL-2

CATTGACACTTGTGCTCCTTGT

TCCTGTAATTCTCCATCCTGCT

 

IL-6

CATGTTCTCTGGGAAATCGTG

TCCAGTTTGGTAGCATCCATC

 
  1. a Mouse derived targeted transcripts. bHouse keeping gene for internal control.