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Figure 1 | Virology Journal

Figure 1

From: A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection

Figure 1

The specificity of the individual primer pairs was evaluated using plasmids and standard sera containing the HBV genome. Type-specific primers AF1/AF2/BA1R (Figure 1a) were used to amplify plasmids/standard sera of HBV genotype A (Lane 1), subgenotype B2 (Lane 2), subgenotype B1 (Lane 3), subgenotype C1 (Lane 4), subgenotype C2 (Lane 5) and genotype D (Lane 6). Lane 7 is the negative control, and lane 8 is the molecular weight standard. The PCR product of genotype A was 709 bp. Type-specific primers of genotype B (Figure 1b), D (Figure 1g) and subgenotype B2 (Figure 1c), B1 (Figure 1d), C1 (Figure 1e) and C2 (Figure 1f) were also used to amplify from these samples. The PCR products of genotype B, subgenotype B2, B1, C1, C2 and genotype D were 308 bp, 382 bp, 576 bp, 510 bp, 195 bp and 671 bp, respectively.

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Virology Journal

ISSN: 1743-422X