Table 1 Primers used in this study to generate overlapping PCR fragments from the genome of D5C1
From: Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1
Fragment designation | Â | Primer sequence (5'-3') | Positionc | Expected product size (bp) |
|---|---|---|---|---|
Le | Fa | ACCAAACAGAGAATCCGTGAG | 1-21 | 2280 |
| Â | Rb | TGGACGATTTATTGCTAAGCTTG | 2258-2280 | Â |
B1 | F | CAAGACTGGAGCAAGCAACT | 2219-2238 | 2003 |
| Â | R | GGAGAGGCATTTGCTATAGG | 4202-4221 | Â |
B2 | F | GGGCTCAGTGATGTGCTCG | 4100-4118 | 1964 |
| Â | R | ATATAGGTAATGAGAGCAGATGTG | 6040-6063 | Â |
B3 | F | AAATAATATGCGTGCCACCT | 5434-5453 | 1661 |
| Â | R | GAACGCAGAGTAGAAAAGAATA | 7073-7094 | Â |
A1 | F | CAAGAACACCTGAATTTTATCCCG | 6686-6909 | 2260 |
| Â | R | TTAGATGCCTTTGGACCTGTTTTA | 8922-8945 | Â |
A2 | F | TGGTTTCACTCAAAATGGTCC | 8876-8896 | 1223 |
| Â | R | ATCCCTTCTGCCATTACCTG | 10079-10098 | Â |
A3 | F | ACCCTTGAGTACCTAAGAGATGA | 9965-9987 | 2116 |
| Â | R | TGTCCCCATAAGCCCAGAT | 12062-12080 | Â |
Tr | F | CTAGGAAGAGCCTTAATTTGAT | 13350-13371 | 1838 |
| Â | R | ACAAAGATTTGGTGAATGACA | 15167-15187 | Â |