Figure 2
From: Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1
Verification of the helper plasmids pCI-NP, pCI-P and pCI-L in BSR-T7/5 cells co-transfected with minigenome pTVT-TGL. Green fluorescence was observed under fluorescence microscopy in minigenome-transfected cells 48 h post-transfection. Panel “TVT-TGL 100×”: Cells transfected with pCI-NP, pCI-P, pCI-L and pTVT-TGL (100×); Panel “TVT-TGL 200×”: Cells transfected with pCI-NP, pCI-P, pCI-L and pTVT-D5C1 (200×); Panel “NC 100×”: Cells transfected with pTVT-TGL (100×) only. UV-light: ultraviolet.