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Figure 1 | Virology Journal

Figure 1

From: Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1

Figure 1

Schematic representation of the minigenome pTVT-TGL and construction of the full-length cDNA plasmid pTVT-D5C1. (A) The reverse genetics vector pTVT7R(0.0); (B) the minigenome pTVT-TGL; (C) the full-length cDNA plasmid pTVT-D5C1. To contruct full-length cDNA plasmid of pTVT-D5C1, eight PCR fragments spanning the following positions were amplified: Le, nt 1 to nt 562 (Eag I); B1, nt 562 (Eag I) to nt 3549 (Sac II); B2, nt 3549 (Sac II) to nt 5450 (Bss HI); B3, nt 5450 (Bss HI) to nt 6601 (Age I); A1, nt 6601 (Age I) to nt 11381 (Mlu I); A2, nt 11381 (Mlu I) to nt 13523 (Afl II); A3, nt 13523 (Afl II) to nt 15106 (Stu I); and Tr, nt 15106 (Stu I) to nt 15198. All the fragments were orderly joined with pTVT7R(0.0). The segment Le followed the T7 promoter; the segment Tr was followed by the hepatitis delta virus (HDV) ribozyme and the T7 terminator. For pTVT-TGL, the fragment was inserted into pTVT7R(0.0) in the reverse direction.

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