Figure 7
From: Efficient inhibition of HIV-1 replication by an artificial polycistronic miRNA construct
Evaluation of off-target effects of miR-ACB. (A) The cell proliferation rate of MT-4 cells with and without miR-ACB. MTT was detected after cells were plated and cultured 48 h at 37 °C. (B) The Stat1 mRNA level of MT-4 cells with and without miR-ACB was quantified by qPCR and normalized to the β-actin mRNAs. (C) Expression profiles of miRNAs in MT-4 with and without miR-ACB. Expression level of miRNAs was measured by qPCR and normalized to that of small non-coding RNA U6B. Error bars in all cases indicate the standard deviation of triplicate cultures.