Figure 4
From: Efficient inhibition of HIV-1 replication by an artificial polycistronic miRNA construct
Screening of the combinations of the basic structural elements. The miRNA complexes were co-transfected with luciferase reporters in 293FT cells. Luciferase activity in cells was measured 48 h post-transfection and normalized to the Renilla luciferase activity. miR-LacZ was used as a negative control. The relative luciferase activity was defined according the ratio of individual miRNA to miR-LacZ. (A) Screening of the combinations of two basic structural elements. (B) Screening of the combinations of three basic structural elements. The combination of miR-CB with miR-A produces 2 varieties of triple miRNA clusters: miR-ACB and miR-CBA. The inhibitory effect of miR-ACB was greater than that for miR-CBA.