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Figure 1 | Virology Journal

Figure 1

From: Efficient inhibition of HIV-1 replication by an artificial polycistronic miRNA construct

Figure 1

Screening of highly effective shRNAs against HIV-1 sequences. (A) The relative virus production (black bars) in a cotransfection assay of indicated shRNAs with HIV-1 infectious clone, pNL4-3. Virus production in the presence of shRNA targeting GFP was used as a negative control (gray bar). Virus production was measured by CA-p24 ELISA and corrected for transfection efficiency by including Renilla luciferase in the transfection assay. The ratio between CA-p24 and Renilla values yielded the relative virus production, which was set at 100% for the negative control. The mean values and standard deviations were obtained from three independent transfections. (B) The 52 shRNA constructs with inhibition efficiency greater than 97% were aligned with 625 HIV-1 full sequences in the Los Alamos HIV Sequence Database. The non-mutation percentage of shRNA target sequence is shown here.

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