Manipulation of immunodominant dengue virus E protein epitopes reduces potential antibody-dependent enhancement

Table 2 WT and cross-reactivity reduced vaccines epitope-specific IgG antibody response

Vaccine	Specific Epitope¹	Endpoint mean²	Endpoint range	% response mean³	% response range³
WT	E	9.8 × 10⁴	<1.0 × 10² -2.9 × 10⁵	100.0	100.0
	EDII_FP	5.9 × 10⁴	<1.0 × 10²-1.2 × 10⁵	66.8	45.3-93.6
RD	E	1.6 × 10⁵	2.9 × 10²-9.7 × 10⁵	100.0	100.0
	EDII_FP	4.3 × 10⁵	7.1 × 10⁴-1.2 × 10⁶	0.3⁴	<1-3.2
ERR	E	1.2 × 10⁵	<1.0 × 10²-5.2 × 10⁵	100.0	100.0
	EDII_FP	9.1 × 10⁴	<1.0 × 10²-1.8 × 10⁵	81.2	58.9-100
RDERR	E	4.6 × 10⁴	<1.0 × 10²-3.1 × 10⁵	100.0	100.0
	EDII_FP	7.1 × 10⁵	<1.0 × 10²-2.3 × 10⁶	<1⁴	<1

¹ Epitopes targeted by specific antibody populations. E denotes antibodies recognizing all E-protein epitopes. EDII_FP denotes broadly cross-reactive WT epitopes of the E domain II fusion peptide.
² Endpoint titers determined with WT and epitope knock-out antigens, thus representing immunoglobulins not recognizing epitopes targeted by the knock-out antigen [19] (see methods).
³ Percentages below limit of detection are expressed as <1.
⁴ The percent IgG recognizing these epitopes was significantly lower than for WT immunized mice as determined by a non-parametric Kruskal-Wallis test and Dunn’s multiple comparison tests.

ISSN: 1743-422X