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Figure 1 | Virology Journal

Figure 1

From: Prediction and identification of mouse cytotoxic T lymphocyte epitopes in Ebola virus glycoproteins

Figure 1

Identification of H-2d-restricted epitopes in SEBOV-GP. (A, B) BALB/c mice were immunized with Ad5-GPS twice, at an interval of 4 weeks. Splenocytes were harvested 10 days after the second immunization and re-stimulated in vitro with the predicted peptides from SEBOV-GP for use in IFN-γ ELISPOT assays. RF was observed to induce strong IFN-γ-specific spot forming. A negative control without peptide was included. (C, D) To further confirm the peptide identified in the ELISPOT assay, BALB/c mice were immunized with Ad5-GPS or Ad5-EGFP (as a control) twice, splenocytes were re-stimulated in vitro with RF or without peptides and the responding CD8+ T cells were visualized by intracellular IFN-γ staining.

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