Figure 3
From: Murine gammaherpesvirus 68 glycoprotein 150 does not contribute to latency amplification in vivo
Immune response after vaccination with gp150-containing exosomes. Mice were vaccinated twice (day 0 and 14) with gp150-containing exosomes (293/gp150) or as a control, with exosomes prepared from untransfected 293 cells (293). Nineteen days after the second vaccination (day 33), sera were analyzed by ELISA for the presence of anti-gp150 antibodies (panel A). For comparison, antibody levels of MHV-68 infected mice (> 2 weeks after infection) are shown. The presence of gp150-specific T-cells was detected by re-stimulation of splenocytes with exosomes (+/− gp150) and subsequent quantification of activated T-cells by IFN-γ-ELISPOT (panel B). Data shown are means + SD derived from eight (293 exosomes), seven (293/gp150 exosomes) and two (MHV-68 infected) mice, respectively. The Student’s t-test (unpaired) was used for statistical analysis