Figure 2
From: Murine gammaherpesvirus 68 glycoprotein 150 does not contribute to latency amplification in vivo
Characterization of exosomes. Exosomes were analyzed for overall gp150 content by Western Blot (panel A) and for gp150 surface content by FACS analysis (panel B). A) Gp150 was detected in purified exosomes and total cell lysates with polyclonal rabbit anti-MHV-68 antibody. Marker sizes (in kD) are indicated on the left. B) Gp150 on the surface of exosomes was detected by binding of exosomes to flow cytometry Protein G antibody binding beads coated with polyclonal rabbit anti-MHV-68 antibody. Binding of exosomes was visualized by staining for the exosome marker CD63 using a PE-conjugated anti CD63 monoclonal antibody. Solid black line: beads only; dotted black line: beads + 293 exosomes; grey histogram: beads + 293/gp150 exosomes.