Figure 1

gp150 does not contribute to latency amplification. A) and D) Spleen weights, B) and E) Ex vivo reactivation of splenocytes and C) and F) Viral genomic load in the spleen. C57BL/6 mice (A-C) or Balb/c mice (D-F) were infected i.n. with 5×10⁴ PFU. At day 17 after infection, spleens were harvested and the spleen weights were taken (the spleen weight of uninfected mice of the same age was in the range of 80 to 120 mg; data not shown). Single splenocyte suspensions were prepared and analyzed in the ex vivo reactivation assay or used for DNA isolation for real time PCR analysis. Data shown in panels A) and D) are means + SD, in A) from 6 mice compiled from two independent experiments and in D) from three mice from a single experiment. Data shown in panel B) are means + SEM, derived from two experiments. Data shown in panel E) are derived from a single experiment. In each experiment, splenocytes from 3 mice per group were pooled. The dashed line in panels B) and E) indicates the point of 63.2% Poisson distribution, determined by nonlinear regression, which is used to calculate the frequency of cells reactivating lytic replication. In panels C) and F), each symbol represents an individual mouse and the bars represent the mean. The data in C) are compiled from 2 independent experiments, the data in F) are from a single experiment.