Figure 6From: PA from an H5N1 highly pathogenic avian influenza virus activates viral transcription and replication and induces apoptosis and interferon expression at an early stage of infectionTUNEL staining and Caspase 3, 8, and 9 activities of C-PA- and WSN-infected cells. A. TUNEL assays were performed on C-PA- and WSN-infected MDCK cells at the indicated time after infection. The mean and standard deviation (error bar) of the number of TUNEL-positive cells counted from 3 random fields were calculated. B. The activities of caspases 3, 8, and 9 in C-PA- and WSN-infected MDCK cells were measured 10 hr pi. Caspase activity is expressed as the fluorescence intensity/total cellular protein (mg) calculated from 3 independent measurements. Mock: mock-infected MDCK cells. Statistical significance was evaluated with Student’s t-test. *p < 0.05, ***p < 0.005, ****p < 0.0005.Back to article page