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Figure 6 | Virology Journal

Figure 6

From: PA from an H5N1 highly pathogenic avian influenza virus activates viral transcription and replication and induces apoptosis and interferon expression at an early stage of infection

Figure 6

TUNEL staining and Caspase 3, 8, and 9 activities of C-PA- and WSN-infected cells. A. TUNEL assays were performed on C-PA- and WSN-infected MDCK cells at the indicated time after infection. The mean and standard deviation (error bar) of the number of TUNEL-positive cells counted from 3 random fields were calculated. B. The activities of caspases 3, 8, and 9 in C-PA- and WSN-infected MDCK cells were measured 10 hr pi. Caspase activity is expressed as the fluorescence intensity/total cellular protein (mg) calculated from 3 independent measurements. Mock: mock-infected MDCK cells. Statistical significance was evaluated with Student’s t-test. *p < 0.05, ***p < 0.005, ****p < 0.0005.

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