Table 2 Primers for viral screening

HRSV

RT-PCR primers

HRSV-AF

F

669-695

ctgtgatagarttccaacaaaagaaca

[8, 9]

HRSV-AF

F

718-745

agttacacctgcattaacactaaattcc

[8, 9]

HRSV-BN

N

435-458

ggctccagaatataggcatgattc

[8, 9]

HRSV-BN

N

480-508

tggttattacaagaagagcagctatacacagt

[8, 9]

MGB probes

HRSV-A TP

F

697-715

cagactactagagattacc

[8, 9]

HRSV-B TP

N

461-477

tatcatcccacagtctg

[8, 9]

INFLUENZA A

AM_FW155

M

151-174

catggaatggctaaagacaagacc

[7]

AM_RV397

M

374-397

aagtgcaccagcagaataactgag

[7]

INFLUENZA B

BHA_FW188

HA

188-209

agaccagagggaaactatgccc

[7]

BHA_RV347

HA

324-347

ctgtcgtgcattataggaaagcac

[7]

PARAINFLUENZA

PIV1FW

HN

748-768

ccttaaattcagatatgtat

[4]

PIV1RV

HN

1206-1225

gataaataattattgatacg

[4]

PIV2 FW

HN

803-822

aacaatctgctgcagcattt

[4]

PIV2 RV

HN

1291-1310

argtcagacaatgggcaaat

[4]

PIV3 FW

HN

762-781

ctgtaaactcagacttggta

[4]

PIV3 RV

HN

1220-1239

tttaagcccttgtcaacaac

[4]

PIV4 FW

P

531-552

gaaagaggcttgggttacaca

[21]

PIV4 RV

P

1147-1168

gctcttatcacagtctccaaa

[21]

HMPV

HMPV_N3F

N

357-379

gagaagagctgggtagaa

[6]

HMPV_N3R

N

716-733

caaacaaactttctgct

[6]

CORONA

PanCoV FW

RdRp

13717-13739*

acwcarhtvaayytnaartaygc

[5]

PanCoV RV

RdRp

13948-13967*

tcrcayttdggrtartccca

[5]

ADENO

AdFW

Hexon

21-46

gccscartggkcwtacatgcacatc

[22]

AdRV

Hexon

301-322

cagcacsccicgratgtcaaa

[22]

RV

RV_5UTR_FW

5'UTR

186-203

caagcacttctgtttccc

[23]

RV_5UTR_RV

5'UTR

566-584

cacggacacccaaagtagt

[23]