The expression of heterogous gene under the HHV-6 promoter in recombinant VZV-infected cells. (A) The HHV-6MIEpin1-DsRed-vOka-BAC DNA was transfected into MRC-5 cells. The infectious virus, reconstituted from the HHV-6MIEp-DsRed-vOka-BAC DNA, caused typical cytopathic effects along with green fluorescence and red fluorescence at 5 days post-transfection. (B) The HHV-6MIEpin1-DsRed-vOka-BAC-infected MRC-5 cells and vOka-BAC-infected MRC-5 cells were lysed in sample buffer, and subjected to Western blot analysis. Blots were reacted with an anti-DsRed mAb or anti-VZV gB Abs. The position and molecular mass in kDa of marker proteins are indicated at the left. Lane 1: vOka-BAC-infected MRC-5 cells, lane 2: HHV-6MIEpin1-DsRed-vOka-BAC-infected MRC-5 cells.