Confirmation of the insertion of HHV-6MIEp-DsRed into the vOka-BAC genome by Southern blot. (A) The HHV-6MIEpin1-DsRed-vOka-BAC DNA and the vOka-BAC DNA were digested with BamHI, loaded onto a 0.5% agarose gel, and separated by electrophoresis. The DNA fragments were visualized with a UV transilluminator. Arrowheads indicate the band shift following transposition. Each DNA size is shown on the right side of the panel. (B) The blots were hybridized with ORF12, ORF13, DsRed, or HHV-6MIEp probes. Bands were detected by the Enhanced Chemiluminescence (ECL) Direct Nucleic Acid Labeling and Detection System. Lane M: size markers, lane 1: vOka-BAC DNA, lane 2: HHV-6MIEp-DsRed-vOka-BAC DNA.