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Figure 3 | Virology Journal

Figure 3

From: Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression

Figure 3

Comparison of promoter activity in deletion mutants of HHV-6MIEp and HHV-6MIEp-in1. (A) Schematic representation of 5'-deletion mutants of the HHV-6MIEp (black arrows) and of the HHV-6MIEp-in1, which is the HHV-6MIEp including intron 1 (white arrows). The name and size of each promoter are shown at the right (without intron 1) and left (with intron 1). Putative transcription factor-binding sites, predicted by TFSEARCH (http://www.cbrc.jp/research/db/TFSEARCH.html), are shown. (B) Luciferase assays were performed in various cell lines. The mean fold-activity relative to that of the blank pGL3-basic plasmid is indicated by the horizontal bars. The standard deviation for three independent experiments is indicated. The activities of CMV MIEp, deletion mutants of the HHV-6MIEp, and deletion mutants of the HHV-6MIEp-in1, are indicated by gray, black, and white bars, respectively. One asterisk indicates that the P value is < 0.05 and two asterisks indicate that the P value is < 0.01 in comparison with the CMV MIEp, as determined by Student's unpaired two-tailed t-test.

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