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Figure 2 | Virology Journal

Figure 2

From: Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression

Figure 2

The activity of the HHV-6 MIE promoter including intron(s) of the IE gene. Schematic representation of the HHV-6 MIE promoter variants, including introns 1-4 of the IE1 gene, used for analysis of the introns. The numbers indicate the nucleotide position from the 5' end of mRNA encoded by the IE1 gene. The +1 indicates the 5' end. The name and size of each promoter used here are shown at the right. (B) Luciferase assays were performed after transfection in various cell lines (Molt-3, Jurkat, SupT-1, MRC-5, Mewo, and U373). The mean fold-activity of each HHV-6 MIE promoter variant relative to that of the blank pGL3-basic plasmid is shown by the horizontal bar. Standard deviation for three independent experiments is indicated. One asterisk indicates that the P value is < 0.05 and two asterisks indicate that the P value is < 0.01 in comparison with HHV-6MIEp-d2 (without intron 1), as determined by Student's unpaired two-tailed t-test.

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