Figure 5

CD23^hi cells and IL6⁺ cells are derived from CD27⁺ memory B cells. A. PBMC were FACS-sorted into CD27⁺ (memory) and CD27^- (naïve) B cells. Sorting strategy and purity of cells after sorting are shown. B. Sorted CD27⁺ and CD27^- B cells were exposed to EBV. Cells were harvested on day 5 and examined for expression of CD23 (FITC) and intracellular IL6 (APC) by flow cytometry. Percentages represent IL6⁺ or CD23^hi cells of CD27⁺ or CD27^- B cells.