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Figure 5 | Virology Journal

Figure 5

From: Expression and characterization of recombinant VP19c protein and N-terminal from duck enteritis virus

Figure 5

Analysis of solubility and purification of recombinant protein by SDS-PAGE. M, wide molecular weight protein marker; 1, the supernatant of induced pET32a-VP19c after sonication; 2, the pellet of induced pET32a-VP19c after sonication; 3, the inclusion bodies of rVP19c by rough extraction; 4, the purified rVP19c by Ni-NTA affinity column; 5, the supernatant of induced pET32a-VP19c(N) after sonication; 6, the pellet of induced pET32a-VP19c(N) after sonication; 7, the inclusion bodies of rVP19c(N) by rough extraction; 8, the purified rVP19c(N) by Ni-NTA affinity column.

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