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Table 3 Quantitative detemination of virus titers

From: Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures

Virus

EM particles/ml

PCR genomes/ml

Infectivity FFU/ml

log10 difference PCR to EM

log10 difference FFU to EM

log10 difference FFU to PCR

EBOZV Mayinga

2.5 × 108

8.3 × 1010 ± 3.2 × 109

1.0 × 105

+2

-3

-5

EBOSV Maridi

7.5 × 108

8.6 × 1010 ± 5.4 × 109

1.0 × 103

+2

-5

-7

EBOSV Boniface

8.0 × 108

5.9 × 1010 ± 3.4 × 109

1.0 × 104

+2

-4

-6

MARV Ravn

4.0 × 108

1.3 × 108 ± 1.8 × 106

1.0 × 105

0

-3

-3

MARV Ozolin

5.0 × 109

1.6 × 109 ± 1,1 × 108

1.0 × 106

0

-3

-3

MARV Musoke

3.0 × 109

5.8 × 108 ± 2.7 × 107

1.0 × 105

-1

-4

-3

LASV Josiah

1.0 × 106

2.2 × 107 ± 4.7 × 106

1.0 × 104

+1

-2

-3

RVFV ZH548

2.5 × 109

2.9 × 1011 ± 4.3 × 109

1.0 × 106

+2

-3

-5

DENV-1 231

3.0 × 109

4.6 × 1010 ± 4.3 × 109

1.0 × 106

+1

-3

-4

YFV Asibi

4.0 × 108

8.2 × 109 ± 1.7 × 108

1.0 × 108

+1

0

-1

CCHFV 10200

2.5 × 107

1.0 × 106 ± 6.1 × 105

1.0 × 106

+1

-1

-2

  1. EM: calculated from 10 grid squares, qPCR: average and standard deviation of triplicate qPCR results (8 qPCR results for CCHFV), FFU: mean of three titrations.