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Table 2 Primers and probes

From: Quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures

Virus

Oligomer

Sequence 5`to 3'

CCHFV

CCFM FP

TCACCTTAGAGGAGGACACTGAAGG

 

CCFM RP

CTCTTTTGAAAGAAAGTGTCATCACAATC

 

CCHF M LNA

6FAM - TGGTGTAA GA GAAA TC - BBQ

LASV

LAS FP

YAACTCTGCATTYTTCACATCCC

 

LAS RP

TGGGMAACCTAAGYTCACAGCA

 

LAS P

6FAM - ACCACTCCATCTCTCCCAGCC - TMR

  1. LNA Nucleotides in bold, IUB code used Y for C/T, M for A/C, dyes and quenchers used: 6FAM =, 6-Carboxyfluorescein BBQ = Black Berry Quencher, TMR = Tetramethylrhodamin