Table 1 Description of antibodies used for determination of FFU
Virus | Primary antibodies | Secondary antibodies |
|---|---|---|
LASV | Mouse serum Anti-Lassa virus (NP), (in-house) | FITC-conjugated Rabbit Anti-Mouse Immunoglobulins, (DAKO Cytomation, Denmark) |
EBOZV/EBOSV | Mouse serum Anti-Ebola virus (NP), (in-house) | FITC-conjugated Goat Anti-Mouse IgG, (Jackson ImmunoResearch, Baltimore, USA) |
MARV | Human patient serum | FITC-conjugated Goat Anti-Human IgG (FC specific), (Sigma-Aldrich Ltd., UK) |
CCHFV | Rabbit serum Anti-CCHFV (NP), (in-house) | FITC-conjugated Goat Anti-Rabbit IgG (H+L), (Jackson ImmunoResearch, Baltimore, USA) |
RVFV | Mouse polyclonal antibodies (provided by Michele Bouloy, Pasteur Institute, Paris). | FITC-conjugated Rabbit Anti-Mouse Immunoglobulins, (DAKO Cytomation, Denmark) |
DENV 1 | Mouse monoclonal to Dengue Virus E glycoprotein, (Abcam, Cambridge, UK) | FITC-conjugated Goat Anti-Mouse IgG, (Jackson ImmunoResearch, Baltimore, USA) |
YFV | Mouse Monoclonal to Yellow Fever Virus, (Abcam, Cambridge, UK) | FITC-conjugated Goat Anti-Mouse IgG, (Jackson ImmunoResearch, Baltimore, USA) |