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Table 5 Comparison of expression changes of some selected genes between microarray and qRT-PCR

From: Japanese encephalitis virus infection induces changes of mRNA profile of mouse spleen and brain

Gene name

Gene description

Fold change

  

Microarray

qRT-PCR

Spleen

   

Cxcl10

chemokine (C-X-C motif) ligand 10

3.418

3.375 (±1.065)

Ifng

interferon gamma

2.229

3.062 (±0.507)

Gzmb

granzyme B

6.202

7.285 (±0.311)

Gzma

granzyme A

4.236

3.815 (±0.127)

Prf1

Porferin 1

1.820

2.287 (±0.643)

Irf7

interferon regulatory factor 7

3.378

4.926 (±0.309)

Il1r2

Interleukin 1 receptor, type II

2.069

2.078 (±0.105)

Cd163

CD163 antigen

0.060

0.083 (±0.017)

Brain

   

Cxcl10

chemokine (C-X-C motif) ligand 10

1760.024

13.492 (±1.690)

Ccl2

chemokine (C-C motif) ligand 2

1387.794

188.549 (±8.931)

Ccl4

chemokine (C-C motif) ligand 4

336.804

61.007 (±3.735)

Casp3

Caspase3, apoptosis-related cysteine peptidase

2.119

1.207 (±0.073)

Casp4

caspase4, apoptosis-related cysteine peptidase

77.077

19.969 (±0.050)

Socs1

suppressor of cytokine signaling 1

28.609

11.182 (±0.845)

Socs3

suppressor of cytokine signaling 3

36.912

4.672 (±0.464)

Cd163

CD163 antigen

0.066

0.065 (±0.012)