A rapid method to screen putative mRNA targets of any known microRNA

Table 1 Putative target mRNAs of HCMV miR-UL112-1 identified by hybrid-PCR

Putative target mRNAs		Number of clones	In 3'UTR	Complementary to Seed Region	Predicted by TargetScan	Repeoted before
mRNA encoded	Accession No.
HCMV immediate early protein (IE72)^a	M26973.1	1	+	+		+
HCMV UL17/18 ^a	AC146906.1	1		+
Homo sapiens heat shock protein, alpha-crystallin-related,B6	NM_144617.1	8	+
Homo sapiens CCAAT/enhancer binding protein (C/EBP)	NM_005195.3	5	+	+	+
Homo sapiens NADH dehydrogenase subunit 5 (MTND5)	AF339085.1	2	+	+
Homo sapiens microfibrillar-associated protein 1 (MFAP1)	NM_005926.2	2		+
Homo sapiens mRNA for putative NFkB activating protein ^a	AB097011.1	1	+	+
Homo sapiens interleukin 32 ^a	NM_001012631.1	1	+
Homo sapiens ribosomal protein S18	NM_022551.2	6
Homo sapiens ribosomal protein L7a ^a	BC032533.1	12	+	+
Homo sapiens spermine oxidase	NM_175842.1	3	+
Homo sapiens transportin 1 ^a	NM_002270.3	3	+	+	+
Homo sapiens HSPC193	NM_001145104.1	1	+
Homo sapiens z-cop	AF086911.1	1	+	+
Homo sapiens zinc finger protein 36 ^a	NM_004926.2	4	+	+

Note: Genes conformed to the descriptions were marked by "+"in columns. Genes marked by "a" were validated by luciferase reporter assays.

ISSN: 1743-422X