Figure 4
From: A rapid method to screen putative mRNA targets of any known microRNA
Identification of seven validated target genes among hybrid-PCR products with different initial annealing temperature. Seven validated target sequences (including IE72) were identified among those hybrid-PCR products by an additional amplification with specific primers of target sequence. M, DL2000; lane 1, negative control; lane 2, mRNA of HCMV IE72; lane 3, mRNA of zinc finger protein 36; lane 4, mRNA of transportin 1; lane 5, mRNA of ribosomal protein L7a; lane 6, mRNA of interleukin 32; lane 7, mRNA for putative NFkB activating protein; lane 8, mRNA of HCMV UL17/18.