Results of hybrid-PCR. (A) Hybrid-PCR was carried out as described. Product of hybrid-PCR (PmiR-UL112-1) and mRNA-derived cDNA (cDNA) were electrophoresis on 3% agarose gel with DL2000 alongside. (B) Partial chromatogram of clone B29, which was identified containing HCMV IE72 specific sequence. Sequence of miR-UL112-1 hybrid-primer was indicated in red box, and inner primer binding site was indicated in green box. PolyA sequence was down lined in black.