Figure 1

PCR detection of MCMV genomic DNA in fetal organs and placentas from pregnant mice whose placentas were injected with MCMV, 3% FCS DMEM, or without injection. Primers from exon 4 of the MCMV IE gene (GenBank accession number M11788) were used, yielding a 700-bp product. A: Lanes 1-4, DNA from the fetal organs or the placentas of pregnant mice whose placentas were injected with MCMV; Lane 5, DNA from the fetal organs or the placentas of pregnant mice whose placentas were injected with 3% FCS DMEM; Lane 6, DNA from the fetal organs or the placentas of pregnant mice without placental injection; Lane 7, DNA from virus-containing culture fluid. B: DNA sequence of PCR products from organs or placentas with placental MCMV injection.