GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease

Table 3 Primers for Semi-quantitative RT-PCR

Target genes	primers	Fragment length	References
HPRT	5' GTT GGA TAC AGG CCA GAC TTT GTT G 3 GAG GGT AGG CTG GCC TAT GGC T	352 bp	[28]
IL-2	5' TCC ACT TGA AGC TCT ACA G 3' GAG TGA AAT CCA GAA CAT GCC	247 bp
IFN-γ	5' CAT TGA AAG CCT AGA AAG TCT G 3' CTC ATG GAA ATG CAT CCT TTT TCG	267 bp
IL-4	5' GAA AGA GAC CTT GAC ACA GCT G 3' GAA CTC TTG CAG GTA ATC CAG G	240 bp
IL-10	5' CCA GTT TTA CCT GGT AGA AGT GAT G 3' TCT GGT CCT GGA GTC CAG CAG ACT CAA	324 bp

ISSN: 1743-422X