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Table 3 Primers for Semi-quantitative RT-PCR

From: GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease

Target genes primers Fragment length References
HPRT 5' GTT GGA TAC AGG CCA GAC TTT GTT G
3 GAG GGT AGG CTG GCC TAT GGC T
352 bp [28]
IL-2 5' TCC ACT TGA AGC TCT ACA G
3' GAG TGA AAT CCA GAA CAT GCC
247 bp  
IFN-γ 5' CAT TGA AAG CCT AGA AAG TCT G
3' CTC ATG GAA ATG CAT CCT TTT TCG
267 bp  
IL-4 5' GAA AGA GAC CTT GAC ACA GCT G
3' GAA CTC TTG CAG GTA ATC CAG G
240 bp  
IL-10 5' CCA GTT TTA CCT GGT AGA AGT GAT G
3' TCT GGT CCT GGA GTC CAG CAG ACT CAA
324 bp