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Table 2 Primers for cloning PCR

From: GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease

Target genes* Primer code Primers Sequences (5'-3')** Fragment length PCR condition
BoIL-2 BoIL-2 F 5' GAA GGA TCC CAC CTC CTA CTT CAA GCT CTA CG 3' 405 bp 94°C for 60 s, 62°C for 60 s and 72°C for 60 s, 35 cycles
  BoIL-2 R 5' CTA GAA TTC CAA GTC ATT GTT GAG TAG ATG C 3'   
BoGM-CSF BoGM-CSF F 5' CTA GAA TTC GCA CCT ACT CGC CCA CCC AA 3' 378 bp 94°C for 60 s, 62°C for 60 s and 72°C for 60 s, 35 cycles
  BoGM-CSF R 5' TTA CCG CGG CTT CTG GGC TGG TTC CCA G 3'   
VP1 VP1 F 5'GCA CCG CGG ACC ACC TCT GCG GGT GAG TCT 3' 669 bp 94°C for 60 s, 61°C for 60 s and 72°C for 60 s, 35 cycles
  VP1 R 5'GAC CTC GAG CAG AAG CTG TTT TGC GGG T 3'   
  VP1 F1 5'GCA GAA TTC ACC ACC TCT GCG GGT GAG TCT 3'   
  VP1 R1 5'GAC CTC GAG CAG AAG CTG TTT TGC GGG T 3'   
  1. Note: *The GeneBank accession number: BoIL-2 (NM_180997), BoIL-6 (NM_173923), BoGM-CSF (U22385). ** In these primers, sequences for the restriction sites were indicated by underlines.