GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease

Table 2 Primers for cloning PCR

Target genes*	Primer code	Primers Sequences (5'-3')**	Fragment length	PCR condition
BoIL-2	BoIL-2 F	5' GAA GGA TCC CAC CTC CTA CTT CAA GCT CTA CG 3'	405 bp	94°C for 60 s, 62°C for 60 s and 72°C for 60 s, 35 cycles
	BoIL-2 R	5' CTA GAA TTC CAA GTC ATT GTT GAG TAG ATG C 3'
BoGM-CSF	BoGM-CSF F	5' CTA GAA TTC GCA CCT ACT CGC CCA CCC AA 3'	378 bp	94°C for 60 s, 62°C for 60 s and 72°C for 60 s, 35 cycles
	BoGM-CSF R	5' TTA CCG CGG CTT CTG GGC TGG TTC CCA G 3'
VP1	VP1 F	5'GCA CCG CGG ACC ACC TCT GCG GGT GAG TCT 3'	669 bp	94°C for 60 s, 61°C for 60 s and 72°C for 60 s, 35 cycles
	VP1 R	5'GAC CTC GAG CAG AAG CTG TTT TGC GGG T 3'
	VP1 F1	5'GCA GAA TTC ACC ACC TCT GCG GGT GAG TCT 3'
	VP1 R1	5'GAC CTC GAG CAG AAG CTG TTT TGC GGG T 3'

Note: *The GeneBank accession number: BoIL-2 (NM_180997), BoIL-6 (NM_173923), BoGM-CSF (U22385). ** In these primers, sequences for the restriction sites were indicated by underlines.

ISSN: 1743-422X