Cytokine gene relative expression analysis of Semi-quantitative RT-PCR. Density of electrophoretic bands in Figure 6 were analysed by band leader 3.0. Taking the data of HPRT bands as the background, Th1 and Th2 cytokine relative expression were evaluated by comparing the intensities of their PCR products and showed in Figure 7. For Th1 or Th2 cytokine, mRNA levels were the highest inoculated with the last four groups, followed by co-inoculation with signal cytokine and VP1, VP1 and VP1 + CFA group had the same level with former groups. (1: control, 2: BoIL-2, 3: BoGM-CSF, 4: BoIL-2/VP1, 5: BoGM-CSF/VP1, 6: VP1, 7: vaccine, 8: VP1+CFA, 9: IL-2+VP1, 10: GM-CSF+VP1, 11: BoIL-2/GM-CSF/VP1, 12: BoIL-2/VP1+GM-CSF/VP1).