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Table 5 Virus recovery from the chickens intranasally inoculated with each virus

From: H9N2 influenza virus acquires intravenous pathogenicity on the introduction of a pair of di-basic amino acid residues at the cleavage site of the hemagglutinin and consecutive passages in chickens

Inoculated viruses

No. of chickens

Days p.i. (Health status)

Virus recovery (log EID50/g)

   

Brain

Trachea

Lung

Liver

Kidney

Colon

Bloodc

rgY55 (H9N2)

3

3 (sacrificed)

-, -, -b

-, -, ≦ 1.7

-, -, -

-, -, -

-, -, -

-, -, -

-, -, -

rgY55sub (H9N2)

3

3 (sacrificed)

-, -, -

5.5, 5.7, 6.5

-, 6.7, 2.7

-, 2.7, -

-, -, 2.5

-, -, -

-, -, -

rgY55sub-P10 (H9N2)

3

3 (sacrificed)

-, -, -

-, -, 3.3

-, 3.7, 6.0

-, -, 2.5

-, 4.3, 4.5

-, 3.3, 4.5

-, -, -

rgVac1 (H5N1)

3

3 (sacrificed)

-, -, -

-, -, -

-, -, -

-, -, -

-, -, -

-, -, -

-, -, -

rgVac1sub (H5N1)

3

3 (sacrificed)

-, -, 2.7

-, -, -

-, -, 2.5

-, -, -

-, -, -

-, -, ≦ 2.0

-, -, -

rgVac1sub-P2 (H5N1)

2

3 (sacrificed)

-, -

-, -

-, -

-, -

-, -

-, -

-, -

 

1a

3 (sacrificed)

5.0

3.8

5.0

5.2

7.5

5.2

4.3

rgVac1sub-P3 (H5N1)

2a

3 (dead)

5.7, 6.7

5.3, 6.5

4.7, 8.2

3.5, 6.0

7.3, 9.0

5.5, 6.5

NAd

 

1a

3 (sacrificed)

6.3

6.5

6.7

6.5

6.5

6.5

5.8

rgVac1ins (H5N1)

3

3 (sacrificed)

-, ≦ 2.6, -

-, 3.5, -

-,3.3, 3.7

-, -, 3.5

-, 2.7, 3.0

-, 3.0, 2.5

-, ≦ 1.6, 2.8

rgVac1ins-P2 (H5N1)

1a

2 (dead)

3.5

3.4

4.7

3.7

4.8

4.7

NA

 

1a

3 (sacrificed)

3.8

3.7

3.0

≦ 2.0

4.7

≦ 2.0

-

 

1

3 (sacrificed)

-

-

-

-

-

-

-

rgVac1ins-P3 (H5N1)

3a

3 (sacrificed)

3.4, 4.7, 5.5

4.7, 3.5, 4.2

5.5, 5.2, 6.7

4.3, 4.5, 5.7

4.5, 4.7, 5.2

5.3, 4.5, 5.3

3.0, 2.5, 3.5

  1. a Each chicken showed depression.
  2. b 1.5 ≧ (0.5 ≧ for blood samples)
  3. c log EID50/ml
  4. d Not applicable.